春季施肥对多花黑麦草种子生产性能的影响

采用完全随机试验设计,研究了多花黑麦草Lolium multiflorum春季不同时期(分蘖期、拔节期和孕穗期)、不同施肥量对其种子生产性能的影响,结果表明,各处理对种子产量的影响极显著(P<0.01),处理B2[拔节期施肥N∶P∶K=20∶18∶22.5(kg/hm2)]种子产量最高,达到620.1 kg/hm2,比对照增产48.7%。与对照相比,千粒重和小穗数均显著增加。     

化除宿营法建立人工草地研究

化除宿营法建植人工草地产草量高,建植第2年最高干物质产量达到23 270 kg/hm2。化除宿营法与化除免耕法建立人工草地在建植当年没有显著差异,但第2年化除宿营法比化除免耕法增产7 353 kg/hm2(P<0.01)。在草地建植当年和第2年,施肥对地上生物量没有明显影响。化除宿营法建立人工草地宿营强度达到4羊夜即可,第2年平均干物质产量达15 483 kg/hm2。在无人为干预的情况下,蒿类等不可食杂草迅速侵入,土壤肥力越高,扩散速度越快;不可食杂草比例超过50%后,草地基本没有利用价值,地上生物量也不再急剧下降。     

滴灌施肥周期和毛管布设方式对苹果树细根直径时空分布的影响

为探明不同滴灌施肥策略对苹果树细根直径的调控效应,于2019—2021年开展二因素二水平完全组合设计田间试验,毛管布设方式设置一行一管和一行两管,施肥周期设置15 d和30 d,采用微根管原位监测技术,分苹果树正南、正西及东北3个方位和0～19、19～38、38～57、57～76 cm不同深度土层,持续观测苹果树活跃生长期内细根直径的动态变化,分析了苹果树细根直径对毛管布设方式和施肥周期的响应。结果表明：细根直径主要集中在0.5～1.5 mm范围内,约占90%,0～0.5 mm和1.5～2.0 mm直径的细根占比很少。在夏季之前,直径≤1.0 mm的细根增多;夏季之后,细根直径加粗,直径>1.0 mm的细根迅速增加。相较于施肥周期15 d,施肥周期30 d在2020年6—11月和2021年4—7月均能增加细根直径;在大部分土层中,施肥周期30 d会增加细根直径,施肥周期15 d会减小细根直径;在2020年正南和东北方向上,施肥周期15 d会减小细根直径,施肥周期30 d会增加细根直径。一行一管较一行两管在2020年和2021年8月均能增加细根直径;在浅中层土壤(0～38 cm土层...     

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Plasminogen activators/Plasmin system plays pivotal role in regulating reproductive functions of mammals. Here, we examined the effects of modification of in vitro fertilization medium (IVF medium) with the addition of tissue‐type plasminogen activator (t‐PA), on bovine embryo development and quality, assessed by quantification of expression of various genes related to metabolism, oxidation, implantation and apoptosis. In addition, plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI) were measured in the spent media. After conventional IVM, 2016 cumulus‐oocyte complexes (COCs) were divided into four groups with modified composition of the IVF medium containing t‐PA and/or its inhibitor epsilon‐aminocaproic acid (control, t‐PA, t‐PA+ε‐ACA, ε‐ACA). Presumptive zygotes were cultured for 8 days in synthetic oviductal fluid (SOF) medium; gene expression studies were carried out on morulae and blastocysts. t‐PA alone significantly suppressed cleavage and blastocyst formation rates, but this effect was neutralized by the addition of ε‐ACA. PAA in the treated group was significantly reduced by ε‐ACA, but without total elimination. Significant differences were detected in the expression of genes related to apoptosis and/or cell cycle arrest (BAX, BCL2L1, KAT2B) between embryos produced in t‐PA‐modified media and controls, giving an overall notion that the inferior developmental competence of treated embryos may be attributed to apoptotic phenomena induced by t‐PA. In conclusion, it appears that excessive t‐PA content in the IVF media, suppresses blastocyst formation rate, possibly due to induction of apoptotic phenomena.     

配方施肥对毛竹林新竹生长及经济效益的影响

通过"3414"施肥试验,研究了不同N、P、K配方施肥对福建三明毛竹林新竹生长及竹林经济产出的影响。结果表明,N、P、K不同配方对新竹的成竹率、新竹胸径与新竹产量以及对竹林经济产出的影响差异显著。14个配方处理中,N2P3K2的新竹数最高,为对照的1.69倍;N2P2K2的新竹胸径和产量均为最大,分别为对照的1.09和1.91倍,且新竹叶片的N、P、K、Mg浓度最为均衡。该试验条件下,施尿素540 kg/hm2、硫酸钾135 kg/hm2、过磷酸钙450~675 kg/hm2,能较为有效地促进新竹生长与产量持续。各配方施肥对毛竹林的增产幅度为18.1%~192.4%,增产效益明显,N2P2K2的竹林产值最高,是对照的2.92倍,资金产出率(产投比)前3位为N2P2K2、N2P3K2和N2P2K3,分别是3.83,3.43和3.26,N素对新竹生长的影响最大,缺N素、P素试验的资金产出率低于无施肥处理。     

沼液施肥对杨树林地土壤微生物量碳氮的影响

沼液是沼气发酵过后的液体残留物,是一种优质的有机肥料。研究了不同沼液施肥对杨树林地土壤微生物量碳氮的影响。结果表明:施用沼液可以提高土壤微生物量碳、氮的含量,微生物量碳、氮含量增加范围分别为4.29%~61.62%和6.08%~76.10%,土壤微生物量碳氮含量随沼液施用量增加逐渐提高。土壤微生物量碳氮呈正相关。土壤微生物量碳氮比随沼液施用量增加逐渐下降,其变化范围为9.92~10.81,土壤微生物量碳氮比与微生物量碳、氮含量负相关。     

南洋楹造林密度与施肥均匀设计试验

【目的】探讨栽植密度和施肥对南洋楹早期生长的影响,在揭示主要栽培因素与单株材积、蓄积量相关关系和回归模型的基础上,建立密度与施肥组合栽培模式,使良种、立地、施肥等技术要素合理配合,为经营南洋楹速生丰产林提供理论依据。【方法】采用均匀设计和随机完全区组设计试验法,对栽植密度和施肥量等4因素6水平按照均匀设计表 U6(64)建立南洋楹高效栽培试验方案;通过 Stepwise分析选择最优回归模型,确定主要栽培因素;采用 Uniform Design Version 3.00拟合林分产量与施肥量回归模型;通过二次响应面回归 Rsreg获得优化施肥量。【结果】不同试验处理的平均树高、平均胸径、单株材积和每蓄积量差异均达到极显著水平( P ＜0.01);影响南洋楹生长最主要的因素是栽植密度,从单株材积和每蓄积量平衡点考虑,最优栽植密度为3 m ×3 m;单株材积与施肥量的二次回归模型： Y3=6.54E－2+5.29E－8X22+3.47E－5X3－4.27E－5X4,复相关系数 R =0.9480,回归方程显著;优化拟合施肥量为：过磷酸钙(作基肥)274 g +尿素(作当年追肥)48 g +复合肥(作次年追肥)250 g,将此优化施肥量代入回归方程求得理论单株材积为0.0604 m3,比试验设计中单株材积最低值0.0443 m3提高36.3%;通过最优栽植密度和优化施肥栽培模式,蓄积量可达47.2905 m3·hm －2,比试验设计中最低值19.4040 m3·hm －2提高143.7%。【结论】南洋楹的速生特性在早期得到显著表达,对不同栽植密度和施肥响应积极,辅以集约栽培技术措施可以较好发掘南洋楹良种和土地的生产潜力。本研究采用均匀设计试验法建立了南洋楹最佳栽植密度与施肥组合模式,从尽可能少的试验次数中揭示出因素对指标的影响大小和规律,并且进行优化拟合设计,缩短了研究周期和提高了试验的准确性。     

Changes in soil macropores: Superposition of the roles of organic nutrient amendments and the greenhouse pattern in vegetable plantations

Organic farming can potentially mitigate soil compaction, which commonly occurs in vegetable plantations, particularly in greenhouses when compared with open‐air systems. Although several studies have addressed the effect of planting patterns on soil pore characteristics, few studies have focused on changes in pore attributes under organic fertilization. We used adjacent fields during green manure planting as a control to compare the differences in soil physicochemical and macropore parameters between 14‐year‐old greenhouse plots and open‐air plots under organic fertilization. Pearson's correlation analysis was performed to analyse the drivers of soil macropore characteristics in vegetable plantations in Jiangsu Province, China. A significant increase in connectivity, fractal dimensions and total macroporosity and a decrease in bulk density after 14 years of organic farming, particularly in the surface layer in the greenhouse, were observed. However, the difference in pores in the plough pan layer was not significant between the treatments. The volume of small pores (50–500 μm) and medium pores (500–1,000 μm) increased significantly (p < 0.05) in both the greenhouse and open‐air systems, showing significantly positive relationships with soil organic matter (SOM) and total nitrogen (TN) content. Large pores (>1,000 μm) showed a clear decrease, especially in open‐air fields, possibly due to the disappearance of the original straw residues in the surface layer. Small and medium pores in the plough layer increased in greenhouse fields, whereas the opposite occurred in open‐air fields. Overall, compared with open‐air systems, long‐term organic greenhouse patterns had a significantly positive effect on soil pore attributes.     

Application of molybdenum fertilizer enhanced quality and production of alfalfa in northern China under non-irrigated conditions

Molybdenum (Mo) is an essential micro-mineral nutrient for plant growth and development. As an important legume forage, alfalfa (Medicago sativa) is frequently planted in marginal lands of northern China where water scarcity and infertile soil are limiting factors for alfalfa production. Mo plays an important role in nitrogen (N) metabolism in general and N fixation in legume species in particular. To evaluate the role of Mo fertilizer in alfalfa quality and production in semi-arid areas of northern China, we conducted a three-year field experiment in Inner Mongolia from 2012 to 2014. Mo fertilizer at the doses of 0, 150 g hm^?2, 300 g hm^?2yr^?1 was applied consecutively across the three years. Application of Mo at 300 g hm^?2yr^?1 significantly increased dry yield of alfalfa in 2014. Crude protein (CP) content of alfalfa without application of Mo exhibited a decline trend from 2012 to 2014. Application of Mo significantly enhanced total CP in 2012 and 2014, and delayed the reduction in total CP across the three years. In contrast to CP, Mo fertilizer had no significant effects on neutral detergent fiber and acid detergent fiber content. In addition, we found that nitrate reductase activity of alfalfa was increased by Mo fertilizer, which may account for the enhanced CP content in alfalfa. These findings highlight that application of Mo fertilizer can be an effective way to improve alfalfa production and quality in semi-arid areas in northern China.